Study on Spectrophotometric Determining Trace Tetracycline by Phase-separation Floatation

摘要

A novel and green method for separating and enriching trace tetracycline (TC) in environment water by phase-separation Floatation has been proposed, the principium was discussed. In this paper, the hydrophobic complex composed of Al(III) and TC was floated into organic phase under the optimal conditions: pH=6.2, the floatation equipment is home-made, The n-propyl alcohol is organic solvent, sodium chloride is separating phase reagent. The data were obtained by spectrophotometry after floatation; The linear regression equation was A=2.5759×10~5 C (mol·L~(-1)) - 0.2716, The linear ranges were from 5×10~(-7) to 7.8×10~(-5) mol·L~(-1), respectively, with the correlation coefficient (r) better than r=0.9998, The average relative recoveries were 100.1% to 102.3%. The limit of detection was 3.86×10~(-8) mol·L~(-1), It can be applied to analyse the trace TC in water sample, the results were better.