Effect of Calcium nutrient on Calcium Distribution and Ultrastructure of cell and chloroplast in Bunching Onion Leaf

摘要

Effect of calcium nutrient on calcium distribution and ultrastructure of cell and chloroplast in Bunching Onion (Allium fistulosum L. var. caespitosum Makino) leaf was studied in sands with 4 Ca~(2+) treatments (0, 40, 80 and 160 mg/L ), in which the solution was Hoagland's. Localization and distribution of calcium and the influence of calcium nutrition on cell ultrastructure were observed by transmission electron microscopy combined with in situ precipitation of calcium with potassium pyroantimonate technique. The results showed that Ca~(2+) were mainly located in cell membrane, vacuoles, envelope of chloroplast and intercellular space in normal cells of bunching onion, however, Ca~(2+) was found to compartment in cytosol in large quantity, and the release of membrane-associated Ca~(2+) to cytosol and wall-associated Ca~(2+) to the intercellular spaces was observed in Ca-deficient leaves. In serious Ca-deficient leaves, Ca~(2+) quantities in cell decreased. Under Ca-deficiency, chloroplast membranes, plasma membranes and nuclear membranes were damaged, and cell walls disassociated, an enlarged space could be found between cells. In serious Ca-deficient leaves, cell compartmentation was destroyed, chloroplast deformed, plasma separated from cell wall, then whole cell constricted.