Comparative Researches on Two Kinds of Staining Method of Acetylcholinesterase Isozymes

摘要

The staining effects of two staining methods of acetylcholinesterase isozymes were compared. One using acetylthiocholine iodide, K_3[Fe(CN)_6] and CuSO_4 was named as AFCu method, and the other one using acetylthiocholine iodide, K_3[Fe(CN)_6] and FeCl_3 was named as AFFe method. The staining effect of former was better. By physiological researches the acetylcholinesterase (AChE, EC. 3.1.1.7), existing in red cells, liver, spleen, nerve and muscle, plays an important role in erveconduction processes in lives. For its close relationships with Alzheimer's disease (AD), close attention to the function of the AChE in the formation and development of lzheimer's disease and the screen of its inhibitors have been paid in the academic world. Otherwise, to meet the need of rapid detection of organophosphorus pesticides and monitoring of environmental pollution, the AChE has also become more and more important. Two staining methods of AChE isozymes will be compared, in order to be helpful to the researches on the AChE. Because the cholinesterase in the serum is called butyrylcholin-esterase (EC 3.1.1.8), which can also use ATCI as its substrate, the pig serum was used to compare the both staining methods of isozymes of AChE here.