Study on Angelica sinensis endophytic fungi and its antibacterial Activity

摘要

Method Endophytic Fungi were isolated from the stem, leaves and root of Angelica sinensis by the surface sterilization method and scoring method. The antimicrobial activity from isolated endophytic fungi were detected using filter paper method with Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, Klebsiella pneumoniae as indicator microorganisms. the partial isolates were identified based on morphological characteristics and classified according to ITS-rDNA sequences analysis. Result A total of 28 endophytic were isolated from the different tissues of the Angelica sinensis, antibiotic activity of the strain AS-R109 and AS-R203 against four indicator microorganisms were the highest, with inhibiting zones diameter of 18, 22, 25, 20 and 20, 22, 19, 23 mm respectively at concentration of 10 mg/mL. With morphology and molecular biology identification, AS-R109 and AS-R203 was determined to belong to the genus for Alternaria sp. and Penicillium sp., respectively.