Is the Identification of SNP-miRNA Interactions Supporting the Prediction of Human Lymphocyte Transcriptional Radiation Responses?

摘要

Genome-Wide Association Studies (GWAS) are of great importance in identifying the genetic variants associated with traits/diseases. Due to the high number of candidate SNPs some filtering techniques are necessary to be applied. The aim of the study was to develop the comprehensive approach allowing for detailed analysis of both SNP-gene and SNP-miRNA-gene relations. We elaborated and optimized the novel signal analysis pipeline improving significantly the results of the analysis on genotype-phenotype interplay. Direct links between genotype results and gene expression levels were enriched by detailed analysis of SNP-miRNA-gene interactions at both mature miRNA structure/seed region and target binding site level. The proposed technique was applied to the data on lymphocyte radiation response and increased by almost 100% number of potential functional SNPs.