Effect of antimycin A on The Glycolytic Flux in Aspergillus niger

摘要

This thesis chose the metabolism of energy and carbon in Aspergillus niger, the citric acid over-producing mycete, as a model system to research the principal physiological and biochemical characteristics. The effects of energy metabolism on the glycolic pathway, and the mechanism of product inhibitory in Aspergillus niger were researched in detail. The relationship between the consistence of intracellular ATP and the glycolytic flux in Aspergillus niger was investigated by adding oxidative phosphorylation inhibitor-antimycin A. When 0.4 mg/L antimycin A was added to the cell cultures, the consistence of intracellular ATP was approximately 31.96% less than that of the control. The specific activity of phosphofructokinase, one of the rate limiting enzymes of the glycolytic pathway, increased by a factor of 0.135 compared with the control. With the specific activity of phosphofructokinase increased, the rate of glucose consumed increased by a factor of 0.137, in comparison of the control. And the rate of citric acid produced increased by 16.15%. The specific activities of hexokinase were not affected by the accretion of antimycin A. These results are the first answer to the fundamental question of what controls the flux through glycolsis in Aspergillus niger.