Enzymatic Synthesis of Both Enantiomers of Ethyl 4-Cyano-3-Hyd roxyb uta noate

摘要

(R)-Ethyl 4-cyano-3-hydroxybutanoate is the key intermediate for the synthesis of liptor used for lowering high cholesterol and triglycerides. Firstly both enantiomers of ethyl 4-cyano-3-hydroxybu- tanoate were prepared by the enzymatic reduction of ethyl 4-cyano-3-oxobutanoate with two whole cells in high ee and high yield. The (R)-enantiomer was obtained in 89.8% yield and 98.5% ee in the catalysis of 20 mM substrate by the strain Bacillus pumilus Phe-C3;;the (S)-enantiomer was achieved in 83.1% yield and 95.4% ee in the catalysis of 10 mM substrate by the strain Klebsiella pneumomiae Phe-E4. Preparative synthesis were carried out on 200-L scale.