Effect of Pyruvate and Lactic Acid on the Development of Porcine Embryos

摘要

This study aimed to investigate the effects of pyruvate and lactic acid on the earlier development of porcine embryos. 5.56 mmol/L glucose in culture medium (NCSU-23) was replaced with 0.2 mmol/L pyruvate and 5.7 mmol/L lactic acid, namely mNCSU-23. Parthenogenetic embryos and nuclear transferred embryos were transferred into NCSU-23 or mNCSU-23 medium according to the experimental design. Parthenogenetic embryos and nuclear transferred embryos were evaluated for the numbers of 5-8 cells stage on Day 2. Blastocyst rates and the numbers of nuclei in the blastocyst were determined on Day 6. From the results, we observed a higher proportion of the embryos reaching 5-8 cells stage at 48h post-activation in mNCSU/NCSU treatment than control during the first part of in vitro culture(IVC) (P < 0.05). In addition, we also observed that the rates of blastocysts formation in mNCSU/NCSU treatments on Day 6 were significantly higher than other treatments, with mNCSU-23/mNCSU-23 treatment having the lowest rates of blastocysts formation on Day 6 (P < 0.05). Our results have demonstrated that replacing glucose with pyruvate and lactic acid during the first part of IVC may be beneficial to the development of the porcine embryos and stepwise culture system(mNCSU-23/NCSU-23) was optimal.