Analyzed the toxicity of Ganaxolide on Phanerochaete chrysosporium by AFLP

摘要

Galaxolide (l,3,4,6,7,8-hexahydro-4,6,6,7,8,8-hexamethyl-cyclopenta-y-2-benzopyran. HHCB) is recognized as a novel contaminant in water and has potential adverse impacts on aquatic organisms. The toxic effect of HHCB on Phanerochaete chrysosporium was investigated by exposure of the fungus in nitrogen-limited culture medium to various concentrations of HHCB. DNA damage of P. chrysosporium by HHCB was detected. Comparing with that in the control, the percent polymorphism under different concentrations of HHCB increased, from 21.4% to 42.9%. In addition, the result of UPGMA (un-weighted pair group method of arithmetic means) dendrogram showed that the Simple Matching Coefficient (SM) was decreased with an increase in the concentrations of HHCB. Thus, as an environmental pollutant, HHCB has the toxic effect on p chrysosporium at molecular level.