Comparative Transcriptional Profiling of Neurospora crassa and Fusarium graminearum in Response to Fungicides

摘要

@@ Many site-specific fungicides have been developed to control plant diseases, and their precious antifungal mechanisms and target protein/enzymes have been determined in most of fungicides. However, effect of fungicides on genome-wide gene expression has not been fully understood. We used Neurospora crassa as a model fungus to identify a subset of genes differentially expressed in response to fungicides such as fludioxonil (stress response MAP kinase activator)，menadione (oxidizing agent), azoxystrobin (mitochondrial complex Ⅲ inhibitor), fluazinam (uncoupler), mepanipyrim (secretion inhibitor), fluconazole (Lanosterol 14-alphademethylase inhibitor), fenpropimorph (C-14 sterol reductase inhibitor), amphotericin B (sterol-binding agent), micafungin (beta-(1, 3)-glucan synthase inhibitor). To select the early response genes by target protein inhibition, growing hyphae were treated with fungicides for 60 min then RNAs were extracted. We selected the candidate genes using microarray analysis and identified the fungicideresponse genes using quantitative real-time PCR.