The Synthesis and Immune Identification of Bisphenol A Artificial Antigen

摘要

Bisphenol A (BPA) belongs to estrogenic substances, which influences the development of genital system in human being. In this paper, 4, 4-bis (4-hydroxyphenyl) valeric acid (BVA), a derivative of BPA, was chosen as the hapten to prepare artificial antigen. An active ester method was used for coupling BPA hapten with keyhole limpet hemocyanin (KLH) and ovalbumin (OVA). Both UV spectra and immunoassay indicated that the two kinds of artificial antigen B VA-KLH and BVA-OVA were synthesized successfully. The artificial antigen of BPA can be used to develop monoclonal antibody of anti- BPA.