Isolation And Characterization Of A Strain Of Pseudomonas Stutzeri For Coal Biodesulfurization

摘要

A strain of Pseudomonas stutzeri named LH42 was isolated from the petroleum-contaminated soil collected from Liaohe Oil Field, Liaoning, China. The strain was capable of degrading dibenzothiophene (DBT). The bacterium was motile, rod-shaped, and grew optimally at 30°C and initial pH of 7.0. The morphological, physiological characterizations and analysis based on 16S rRNA sequence indicated that this strain was most closely related to Pseudomonas stutzeri (>99% similarity in gene sequence). Coal biodesulfurization of this strain was processed. And a total sulfur removal of 41.5% and organic sulfur removal of 92.9% was obtained within 25 days. Scanning electron microscope (SEM) analysis revealed that the strain was closely attached on the coal surface and the coal was markedly etched.