Application of Bioartificial Liver Reactor with Modified Membrane in Drug-Screening

摘要

The objective is to observe the advantage of the poly(vinylidene difluoride)-modified (PVDF-modified) bioartificial liver (BAL) reactors in the drug screening of Chinese herbs quercetin and resveratrol. The active ingredients of quercetin (0.1 mmol/L) was used to acted on rat hepatic cells for 12 hours both in the PVDF-modified and in the unmodified bioartificial liver reactors, then cisplatin (0.2 mmol/L) was added for 24 hours. Another Chinese herb resveratrol (0.1 mmol/L) was used with the same treatment. Finally, the synthesis of urea and albumin, the activities of lactate dehydrogenase (LDH) and aminotransferase (AST) were detected in these two cultured hepatocytes. Significant difference of the synthesis of urea and albumin was obtained between the PVDF-modified and the unmodified BAL reactors, and a less significant difference was for the activities of LDH and AST. The results showed that the PVDF-modified BAL reactor was more sensitive than the unmodified in liver toxicity and in activity of the liver-protective medicine. Hence, the PVDF-modified BAL reactor is more suitable for clinical drug screening.