Effects of polychlorinated biphenyl(PCB101) and its hydroxylated metabolites on proliferation of MCF-7 human breast cancer cells

摘要

Polychlorinated biphenyls and their hydroxylated metabolites become more and more concerned for their endocrine disruption. At the presented study, experiment was designed to obtain the disruption effect of PCB101 and its hydroxylated metabolites. The proliferation of MCF-7 human breast cancer cells was detected at different exposed concentration and time period. MCF-7 cells were cultured with the concentration of PCB101 and two of its hydroxylated metabolites at 5×10~(-9), 5×10~(-8), 5×10~(-7), 5×10~(-6)mol/L. And the concentrations of 17β-Estradiol(E2) were 2×10~(-9), 2×10~(-8), 2×10~(-7), 2×10~(-6)mol/L respectively. And the proliferation rate was detected after 24h, 48h, 72h, 96h exposure. Ethanol was used as solvent control. The results indicated that PCB101 and its hydroxylated metabolites had no effect on MCF-7 cell proliferation at designed concentrations and exposure time.