Simultaneous separation and determination of Vanillin and o-Vanillin by CE compared with HPLC

摘要

A method for the simultaneous separation and determination of vanillin and o-vanillin by capillary electrophoresis (CE) was developed. The influence of type, concentration and pH of running buffer, and applied voltage on separation was investigated. Under the condition of 50 mmol/L horax-150 mmol/L disodium hydrogen phosphate (pH 7.5) and applied voltage of 15 kV, the vanillin and o-vanillin were separated in less than 6 min. The method was validated in terms of accuracy, precision and linearity and proved to be robust.