Synthesis and Spectral Properties of pH Fluorescent Probes Based on Distyryl Ketone

摘要

Five phenolic hydroxyl-substituted distyryl ketones had been synthesized, characterized and evaluated as pH-dependent fluorescent probes for basic conditions. PH-Sensitive fluorescent probes have been widely used in analytical chemistry, bioanalytical chemistry, medicine and cellular biology [1,2] owing to their simplicity, specificity and sensitivity [3,4]. Moreover, the widely use of fluorescence microscopic imaging technique in cellular biology promotes the development of fluorescent probes. To date, a variety of pH-sensitive probes had been reported [5-10], some also could be used as colorimetric pH-indicators. The pH values could be determined both by solution colour changes through naked eyes and by the fluorescence of the probes. As well known, fluorescence probes designed and synthesized consisted fluorophore and receptor [11]. Distyryl ketones were a excellent fluorophore and special optical properties could be obtained by modification of their substituents [12]. Some probes including distyryl ketone structure had been designed as pH-indicators[13]. The mechanism of these probes to monitor pH value is based on the deprotonation and protonation of phenolic hydroxyl group. Here, we designed and synthesized five phenolic hydroxyl-substituted distyryl ketones and their spectroscopic properties as fluorescent and colorimetric pH-indicators were investigated.