CYCLIC ADENOSINE MONOPHOSPHATE FLUORESCENT PROBE BASED ON FLUORESCENT BRIGHTNESS WIDTH VARIATION

摘要

Disclosed is a cyclic adenosine monophosphate fluorescent probe based on fluorescent brightness width variation, having the structure as shown in formula I: MlotiK1 CNBD-N-linker1-cpEGFP-linker2-MlotiK1 CNBD-C, wherein MlotiK1 CNBD-N is an N-terminal of MlotiK1 CNBD, and has an amino acid sequence as shown in SEQ ID NO: 3; cpEGFP has an amino acid sequence as shown in SEQ ID NO: 4; MlotiK1 CNBD-C is a C-terminal of MlotiK1 CNBD, and has an amino acid sequence as shown in SEQ ID NO: 5; linker1 is WG, and linker2 is RV.