Purification, analysis of structure of a poiysaccharide from the fruit of Lycium barbarum L.

摘要

A poiysaccharide, coded as LBP5a, was extracted from the Lycium barbarum L. with distilled water. LBP5a was precipitated repeated by ethanol and purified by DEAE-52, Sephadex G-75. The structure of LBP5a was detected by FT-IR spectrometer, Ultraviolet spectrophotometer, experiments of periodate oxidation and Smith degradation. The results show that LBP5a is testified to be homogeneous poiysaccharide, and does not contain nucleic acid or protein. Pyranose with 1->3, 1->4 glycosyl linkages mainly exist in LBP5a.