Determination of 4 Kinds of Estrogenic Compound in fish samples Using Solid Phase Extraction and High Performance Liquid Chromatography

摘要

A method was developed for determination of Ethinylestradiol, Diethylstilbestrol, 4-Nonylphenol and Estradiol benzoate residue in fish samples using solid phase extraction and high performance liquid chromatography. Samples were extracted and filtered for HPLC analysis. The analysis was used gradient elution with acetonitrile-water as the mobile phase. Under the optimized conditions, a method with good reproducibility (1.16%~2.45%) and the recovery (80.0%~92.5%) was developed for target components in spiked fish samples. The calibration curves were linear with correlation coefficient ranged from 0.9970 to 0.9983. The detection limit of the method was ranged from 0.0606 to 0.6066μg/ml.This method was rapid, sensitive and nothing interfered determination of the 4 kind of components.