Extraction of Silk Protein from Middle Silk Gland of B.mori for Preparation of 3-D Scaffold

摘要

In this paper, 3-D scaffolds were prepared using native protein solution extracted from middle silk gland of B.mori silkworm (SS). The distribution of pore in 3-D scaffold was homogeneous, and the pore size decreased with increase in the concentration of SS. By changing the concentration from 1.7%, 3.5% to 7%, the porosity rate of scaffolds was 95%, 94% and 91%, respectively. The water absorbency apparently decreases and the water retention rate increases with increase in the concentration of SS. The scaffolds prepared from SS with concentration of 3.5% and 7% have higher strength, on the contrary, the scaffolds prepared from SS with low concentration 1.7% shows higher elasticity. FTIR spectra indicated that SS mainly adopted p-sheet conformation. This paper proposed one green way to extract silk protein from the silk gland of B.mori silkworm and prepare its corresponding scaffolds. It seems meaning to provide implication to develop silk-based biomaterials.