A novel scaffold from recombinant spider silk protein in tissue engineering

摘要

As a new biomaterial, recombinant spider silk protein has attracted much attention in tissueengineering. The pNSR-16/ BL21(DE3)pLysS strains fermented and produced the recombinantspider silk protein, which was then cast into scaffolds. NIH-3T3 cells were cultivated with extractionsof the scaffolds in vitro. The cytotoxicity of scaffolds was analyzed with a MTT assay. Theperformances of cells adhesion, growth and expression on the scaffolds were observed with SEM, HEstaining and immunohistochemistry. Compared with the control, the extract fluid of materialsculturing the NIH-3T3 cells was not apparently different. NIH-3T3 cells could adhere and grow on thescaffolds and secret FGF-2. The pNSR-16 recombinant spider silk protein scaffolds has satisfactorycytocompatibility and the scaffolds are ideal scaffold material for tissue engineering.