A Critical Role of Redox State in Determining HL-60 Cell Differentiation

摘要

The modifications of intracellular redox balance leads to important cellular changes in many cell types. Here, a causal relationship between redox state and monocytic differentiation induced by isoliquiritigennin(ISL) have been studied in the human acute promyelocytic leukaemia HL-60 cells. The modulations of intracellular reactive oxygen species levels by D, L-buthionine-(S, R) sulfoximide (BSO), and N-acetyl-L-cysteine (NAC) caused inducer- and time-dependent or stage-specific effects on HL-60 cell growth inhibition and differentiation. ISL increased intracellular Superoxide anions (O2~(·-)) that was completely inhibited by BSO and NAC. ISL increased NBT reduction ability and the expressions of surface antigens CD11b and CD14 were completely inhibited by BSO and NAC. These observations suggest a critical role of redox state in determining HL-60 cell behaviour and provide new insights into the complex effects of redox perturbations on the intracellular signalling network.