Use for regulating specific transcriptional activity by HDAC2 during TPA-induced HL-60 cell differentiation

摘要

The present invention relates to the use of 12-O-tetradecanoylphorbol-13-acetate (TPA) -induced leukemia cell differentiation during HDC2 specific transcriptional activity regulation. The human myeloid leukemia cell line HL-60 differentiates into monocytes when treated with TPA. However, the mechanism of differentiation of the cells relative to TPA has not been clarified. We performed ChIP-seq profiling of RNA Pol II, HDAC2, Acetyl H3 (AcH3) and H3K27me3, and analyzed differential chromatin state changes during TPA-induced differentiation of HL-60 cells. We focused on anomalous activated genes that appear to have improved H3 acetylation despite increased HDAC2 collection. We found that in a histone deacetylase activity-independent manner, HDAC2 positively regulates the expression of these genes. In conclusion, the present invention revealed a specific-chromatin state during HL-60 cell differentiation after TPA exposure. As a diacetylase activity-independent pathway, HDAC2 can activate transcription of specific genes through interaction with PAX5. Found out.