Correlative Imaging of Live Biological Cells with a Soft X-ray Microscope and a Fluorescence Microscope

摘要

A laser-plasma soft X-ray microscope which is a combination of a highly intense laser-plasma soft X-ray source and a contact microscopy has been developed. We have proposed a correlative imaging method with the laser-plasma soft X-ray microscope and a fluorescence microscope to observe the same biological cells with the both microscopes at the same time. Live hydrated biological cells and cellular organelles have been observed with the correlative imaging method. Using the information of the cellular organelles obtained with the fluorescence microscope, inner structures obtained with the soft X-ray microscope are identified. Since the spatial resolution of the soft X-ray microscope is much higher than that of the fluorescence microscope, fine structures of the cellular organelles of the live biological cells have been visualized with the correlative imaging.