Hyaluronic acid degradation decreases capillary perfusion in acute hemodilution with pegylated albumin

摘要

This study evaluated the role of endothelial glycocalyx during acute hemodilution with a pegylated plasma expander in terms of microvascular function. An acute hemodilution with 40% blood volume exchange was performed in awake hamsters. The endothelial glycocalyx was degraded by hyaluronidase via intravenous infusion. Polyethylene glycol conjugated with human serum albumin (PEG-HSA) and dextran 2000 kDa (Dx2M) were used in the blood exchange. Microvascular function was studied in a dorsal skinfold window. Mean arterial blood pressure in animals treated with hyaluronidase followed by hemodilution with PEG-HSA was lower compared to animals treated with hyaluronidase and hemodiluted with Dx2M. Functional capillary density (FCD) in the animals infused with hyaluronidase and hemodiluted with PEG-HSA significantly decreased to 67±21% of baseline while FCD in other groups was 92±4% of baseline. The beneficial effects of PEG-HSA were attenuated while Dx2M improved microvascular function during condition of glycocalyx degradation followed by hemodilution.