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Polarized Raman investigations of oriented animal muscle fibers affected by storage time applying a 671 nm diode laser

机译:通过应用671nm二极管激光的储存时间影响的面向动物肌纤维的偏光拉曼研究

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Due to its analytical ability and sensitivity to molecular vibrations, Raman spectroscopy provides valuable information of the secondary structure of proteins. Moreover, polarized Raman spectroscopy is shown to be a useful instrument to investigate the structural changes resulting from the aging and spoilage process of meat. In this work, polarized Raman spectra were measured on oriented cuts of pork and turkey. Fresh meat slices were stored at 5 °C and measured for a consecutive time period of 10 days. A 671 nm microsystem diode laser was used as excitation light source. The laser power at the sample was 50 mW and the integration time of each Raman spectrum was set to 5 seconds. Measurements were performed with a laser beam orientation perpendicular to the long axis of the muscle fibers. In that arrangement, the fibers were aligned either parallel or perpendicular to the polarization direction of the laser source. By using the statistical method of principal components analysis (PCA), a clear separation of the meat samples can be found for fresh meat according to the orientation (parallel or perpendicular) using the first two principal components. During the storage period, this separation subsequently vanishes due to the aging process and due to an increase of the microbial spoilage of the meat surface. For the latter effect, a time-dependent distinction of the Raman spectra is presented as well. Furthermore, specific changes of conformation-sensitive Raman bands were recognized, notably a decrease of the intensities of α-helical protein conformation.
机译:由于其对分子振动的分析能力和敏感性,拉曼光谱提供了蛋白质二次结构的有价值的信息。此外,偏振拉曼光谱被证明是一种有用的仪器,用于研究由肉的老化和腐败过程产生的结构变化。在这项工作中,偏振拉曼光谱法测量了猪肉和土耳其的导向。将鲜肉切片储存在5℃下,并在10天的连续时间内测量。使用671 nm微系统二极管激光器作为激发光源。样品的激光功率为50mW,每个拉曼光谱的整合时间设定为5秒。用垂直于肌纤维的长轴的激光束取向进行测量。在该布置中,纤维并平行或垂直于激光源的偏振方向对齐。通过使用主成分分析(PCA)的统计方法,可以根据使用前两个主成分的取向(平行或垂直)来发现肉样品的清晰分离。在储存期间,这种分离随后由于老化过程而消失,并且由于肉表面的微生物腐败的增加。对于后一种效果,还提出了拉曼光谱的时间依赖性区别。此外,识别α-螺旋蛋白质构象的强度降低的锥形敏感拉曼带的具体变化。

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