Mechanisms Controlling the Sensitivity of Amperometric Biosensors in the Case of Substrate and Product Inhibition

摘要

Special case of amperometric biosensors is investigated in this paper. Processes of substrate as well as product inhibition take place during the operation of these biosensors. The operation of biosensors is modelled by employing non-stationary reaction-diffusion equations containing a non-linear term related to non-Michaelis-Menten kinetics. The equation system is solved numerically using finite difference technique. Apparent Michaelis constant is chosen as a good indicator of biosensor reliability. Its dependency on the substrate and product inhibition as well as the diffusion modulus and the Biot number was investigated.