Microspheres of Chitosan-Bioactive Glass for Application in Orthopedic Surgery. In vitro experiment

摘要

In order to increase the biocompatibility and bioactivity of bioactive glass, a biodegradable therapeutic polymer (Chitosan: CH) has been incorporated into bioactive glass (BG) by freeze-drying to synthesize biocomposite microsphere material for applications in bone surgery. The interfacial links between bioactive glass and chitosan polymer were investigated though Fourier transformed infrared absorption spectroscopy (FTIR). The surface morphology of the biocomposite and the homogeneous dispersion of bioglass particles in the matrix of chitosan polymer were examined by Scanning electron microscopy (SEM). The bioactivities of the BG and of the BG/CH biocomposite microsphere were investigated by soaking the powder samples in a simulated body fluid (SBF). The formation of a biologically active hydroxycarbonate apatite (HCA) layer on the surfaces of BG and also BG/CH biocomposite was detected. ICP-OES method was employed to evaluate the rate of bioactivity of these two biomaterials. The obtained results indicate that the chitosan enhances the bioactivity of bioactive glass. The biocomposite based on bioactive glass and chitosan polymer have an excellent ability to form an apatite layer on its surface. After three days of immersion, FTIR analyses confirm the formation of this layer. The cytotoxicity and cell viability assays were also investigated by the MTT method using osteoblast like cells SaOS_2. The results confirm the absence of toxicity and a good cell proliferation on this biomaterial. However, the addition of chitosan in the glass matrix enhances the cell proliferation of about 5% at 6 days after culturing.