Infochemicals for the chromatographic separation technology

摘要

Chromatographic separation is the most frequently techniques used in research on the infochemicals and chemical analysis of natural products. In this paper, three secondary metabolites were isolated with fat-soluble substances from the black ants, Polyrhachisvicina Roger by normalphase high-performance liquid chromatography (NP-HPLC) and Sephadex LH-20. And their structure were identified as 3-phenyl, 5, 6-hydride, 1, 2, 4-triazole (compound 1), β-sitosterol (compound 2) and 24-ethyl-β-sitosterol (compound 3).