Expression and purification of His-chlFN-a and its antiviral activity assay

摘要

In this paper, Chicken alpha interferon (IFN-a) gene was cloned into pUC19-His expression vector, then the recombinant expression vector was transformed into host bacteria E. coli BL21. The recombinant chicken IFN-a was induced to express by IPTG, then the protein expression was analyzed with SDS-PAGE. Under the condition that the recombinant protein was induced to express with 1 mM IPTG at 37 bC, the expressed protein was inclusion body. His-chlFN-a was purified by Ni-metal chelate affinity chromatography. His-chlFN-a was shown to inhibit the replication of Newcastle disease virus in CEF cells.