Dynamic Self-assembly process of a designed peptide

摘要

In this paper, we reported the dynamic self-assembly process of an half-sequence ionic self-complementarity peptide CH_3CO-Pro-Ser-Phe-Cys-Phe-Lys-Phe-Glu-Pro-NH_2, which could self-assemble into stable nanofibers and formed hydrogel consisting of >99% water. The dynamic self-assembly process was detected by circular dichroism (CD) and atomic force microscopy (AFM). CD spectrum revealed that the mainly contents of the peptide were regular β-sheet structure. The data indicated that though the secondary structure of the peptide formed immediately, the microstructure of the self-assembly process of the designed peptide formed slowly. AFM image illustrated that the self-assembly process was layer-by-layer assembly.