A Novel Human Hormone-peptide NEROFE with Strong anti Cancer Activity

摘要

We report the isolation of a novel Tumor-Cells Apoptosis Factor (Nerofe). We found that cDNA of this protein is expressed mainly in the human thymus and partially in the colon and in the frontal lobe of brain. Immuno histochemical studies localize Tumor-Cells Apoptosis Factor 's (TCApF) to the medulla and Hassal's corpuscles of the thymus gland, which are responsible for the negative selection. It has been found in that TCApF is a naive ligand of T1/ST2 receptor. This fact together with sequence analysis and presence of free TCApF in blood samples allow considering TCApF as a hormone-like protein. Application of TCApF and its short derivative TCApFs to acute myeloid leukemia proliferating cells (U937 premeyloid cells), human breast carcinoma (MCF7), human glioblastoma, human prostate cancer, murine mammary adenocarcinoma and human lung cancer proliferating cells inhibits the proliferation in vitro. In contrast, TCApF doesn't affect the healthy cells. Treatment of mice with induced AML terminates the cancer development and completely eliminates metastasis cell colonies from the bone marrow and the spleen that reduces probability of the cancer return.