Establishment of an efficient in vitro plant regeneration system in chicory ( Cichorium intybus L. cv. puna)

摘要

In order to shorten the reproductive cycle and to speed up the breeding procedure, a very efficient and rapid regeneration system via callus induction and multiple shoot formation was established for Cichorium intybus L. cv. puna. Factors influencing callus induction, shoot regeneration and root development were researched. The result showed that there was significant effect among different mediums and plant growth regulators (PGRs). MS basal medium supplemented with 6 -BA (2.0mg/L)+IBA (0. 2mg/L) was the most effective compared with the others, while the control treatment involving no plant growth regulators produced no shoots at all. When three expl2mg/L) , in a comparison of euphylla, cotyledon and petiole explants, there was no difants excised were cultured on MS medium supplemented with 6-BA (2. 0mg/L)+IB A (0. ference on callus induction frequency of euphylla and petiole (93. 63% and 97. 67% respectively), but there were difference on regeneration frequency and the number of shoots. The cotyledon had the owest regeneration frequency (68%) , euphylla explants had the highest regeneration frequency(96.12%) and produced over three times more shoots (19.32 shoots) than petiole explants (6. 22 shoots). Root development was readily achieved on 1/2 MS medium containing low concentrations NAA or IB A, but NAA was more effective than IBA and resulted in the highest frequency of shoots that rooted (97.07%) and mean number of roots per shoot (4. 03 roots) and mean length of growing root (6. 51 cm) when used at 0. 1NAA mg/L. and over 97% plantlets were successfully obtained in the greenhouse when planted in a sterile vermiculite and sand mixture medium in pot.