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Compact photoacoustic add-on for a reflectance confocal microscope

机译:紧凑型摄影显微镜的光学光声附件

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Reflectance confocal microscopy (RCM) has become a standard method for skin diagnostics. It is based on tightly focusinga continuous laser beam with an objective into the skin and collecting the back scattered light through a pinhole forgenerating images of selected planes to a depth of several 100 micrometers. For enhancing its diagnostic capability, theRCM can be combined with optical resolution photoacoustic microscopy (OR-PAM), providing strong optical absorptioncontrast for melanocytic lesions. We have developed a compact add-on to common optical objectives that is able to detectthe photoacoustically generated transients with high bandwidth, using four piezoelectric elements made of poly-vinylidenefluoride (PVDF). The elements are arranged on four quadrants of a conical surface around the objective, taking advantageof the focusing effect of the slightly curved surface. For taking an image, the pulsed and continuous excitation beams aresimultaneously scanned over the sample using a pair of galvanometric mirrors. Photoacoustic images of the selected planeare then generated for each separate sensing element and are subsequently added in order to achieve an enhanced signalto-noise ratio. Simultaneously recorded back scattered light provides the input for the RCM mode. We present acharacterization of the sensors and provide experimental results on phantoms.
机译:反射率共聚焦显微镜(RCM)已成为皮肤诊断的标准方法。它基于紧密的聚焦一个连续的激光束,具有目标进入皮肤并通过针孔收集后部散射光将所选平面的图像生成到几个100微米的深度。为了提高其诊断能力,RCM可以与光学分辨率光声显微镜(或PAM)组合,提供强光学吸收黑素细胞病变对比。我们已经开发了一个紧凑的附加加载到能够检测的常见光学目标具有高带宽的光声产生的瞬态,使用由聚亚乙烯基制成的四个压电元件氟化物(PVDF)。这些元件布置在圆锥形表面的四个象限上,围绕目标,利用略微弯曲表面的聚焦效果。用于拍摄图像,脉冲和连续的激励梁是使用一对电流镜同时扫描样品。所选飞机的光声图像然后为每个单独的传感元件生成并且随后添加以实现增强的信号 - 噪声比。同时录制的散射光为RCM模式提供输入。我们展示了A.传感器的表征并提供幽灵的实验结果。

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