The extraction and purification of microcystin from microcystic aeruginosa were studied. The efficiency for microcystin extraction was assessed by comparing different polarity extraction agents. Results showed that 60% methanol has the highest efficiency. Ethanol solution could extract microcystins safely, and can be used as a massive security extraction agent. The extraction method and solid phase extraction measure were improved, and suitable flowing phase composition was chosen to detect microcystin-LR.
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