A Minimally Invasive Multifunctional Nanoscale System for SelectiveTargeting, Imaging, and NIR Photothermal Therapy of MalignantTumors

摘要

The anti-EGFR antibody, cetuximab, was labeled with IRDye 800CW fluorescent dye and conjugated to gold nanorods (GNR). GNR with aspect ratio of ～4 and plasmon resonance peak at –785 nm were fabricated for use in these experiments. The IRDye:cetuximab:nanorod conjugate treatment with NIR light selectively heated the GNR and was sufficient to treat cancers. Excitation induced fluorescence of the IRDye 800CW enabling real-time imaging. We characterized and optimized the parameters for the conjugation of the GNR to cetuximab to facilitate active targeting of the nanorods to the site of the tumor. This combination of selective targeting, imaging, and photothermal treating of malignant cells is a viable approach for a variety of squamous cell carcinomas