Effects of the protein in wheat straw on enzymatic hydrolysis

摘要

The article used acid protease to remove the protein in wheat straw, and investigated the influence of wheat straw without the protein to enzymatic hydrolysis. By the single factor experiments, the cellulose degradation rate of wheat straw removed the protein was significantly higher than untreated, in the same conditions of enzymatic hydrolysis. It is shortening 24h of enzymatic hydrolysis time and reducing enzyme dosage 90mg/g. By the orthogonal experiments, the optimal reaction conditions for enzymatic hydrolysis of wheat straw with protein removed is the cellulase dosage 170mg, enzymatic time 56h, solid-liquid ratio 1:20, pH 4.8, the reaction temperature 50°C. Compared to the untreated, the cellulose degradation rate increased by 35.58%.