MINERAL-RICH BAMBOO SALT ALLEVIATES ALLERGIC INFLAMMATORY SYMPTOMS AND REDUCES CISPLATIN-MEDIATED VESTIBULO-TOXICITY IN MICE

摘要

Purple bamboo salt (PBS) is a specially processed salt according to the traditional recipe using normal solar salt and bamboo etc in high temperature. It has been used as a folk medicine for the purpose of prevention and treatment of various diseases in Korea. First of all, we investigated the effect of PBS on allergic reaction. PBS inhibited the ear swelling response and histamine release induced by compound 48/80. PBS inhibited the passive cutaneous anaphylaxis by oral administration. Second, we evaluated the anti-inflammatory effect of PBS on phorbol 12-myristate 13-acetate (PMA) plus calcium ionophore A23187-induced proinfiammatory cytokines production from mast cells. PBS product, Powerdental also exhibited a significant antibacterial activity. PBS decreased the PMA plus A23187-induced interleukin (IL)-1 , IL-6, and tumor necrosis factor- production and mRNA expression. Third, we investigated the effect of PBS product, Pilopool on performance of forced swimming test (FST). The immobility time was decreased in Pilopool-fed group in comparison with control group on FST. In blood serum, the contents of creatine kinase and lactic dehydrogenase were decreased and the contents of glucose and total protein were increased. Finally, we investigated the effect of PBS on cisplatin-induced apoptosis of auditory cells. We demonstrated that apoptosis of auditory cells induced by cisplatin was inhibited by treatment with PBS. Activation of caspase-3, caspase-8, and caspase-9 was observed within cisplatin-treated auditory cells. PBS also inhibited the activation of caspase-3, caspase-8, and caspase-9. Collectively, our results suggest that PBS importantly contributes to the prevention or treatment of allergic inflammatory diseases and predict a potential benefit of PBS as an immune regulator. In addition, our findings indicate that blocking a critical step by PBS in apoptosis may be useful strategy to prevent harmful side effects of cisplatin ototoxicity in patients with having to undergo chemotherapy.