Study of Parameters Affecting the Level of Ultrasound Exposure with In Vitro Set-Ups

摘要

Ultrasound (US) exposures are widely used with in vitro cell systems e.g. in stem cell and tissue engineering research. However, without the knowledge of factors affecting the level of US exposure, the outcome of the biological result may vary from test to test or even be misinterpreted. Thereby, some of fee factors affecting in vitro US exposures were studied The level of US exposure was characterized in standard commercial cell culturing plates. The temperature distributions were measured inside the wells using infrared camera and fine wire thermocouples, and pressure and intensity distributions using a laser vibrometer and a schlieren system. The measurements were made at operating frequency of around 1 MHz with varying temporal parameters and powers (up to 2 W of acoustic power). Heat accumulation between the wells varied up to 40 - 50% depending on the location of the well on the plate. This well-to-well variation was be linked to the activity of reporter plasmid on osteoblastic cells. Similar temperature variations within the wells were also measured. Small sub-wavelength change in the exposure distance or, respectively, liquid volume inside the well was found to alter the acoustic field in both magnitude and shape due the standing waves. The gathered data reveals the complexity of the acoustic field in a typical in vitro set-up and gives new information about the environment of the in vitro cells during US exposures. This data may be especially useful when US set-ups are designed or characterized.