Progress in molecular breeding of super hybrid rice by transferring genomic DNA of distant relatives

摘要

The gene pool used in rice breeding programs needs to be expanded by combining regular breeding methods and molecular techniques to make new breakthroughs. Elite maintainer lines, restorer lines, and combinations of super hybrid rice have been developed by genomic DNA transformation with the Spike-stalk Injection Method from distant relatives, including Oryza minuta and Echinochloa crus-galli, into parental lines of hybrid rice. Analysis of molecular markers, DNA sequences, and Southern blotting has revealed that high DNA polymorphism exists between variant lines and their receptors, indicating that a special DNA fragment from distant relatives may be integrated into the genome of rice. Therefore, transformation of genomic DNA from distant relatives to the plant of a target receptor may open an avenue for creating new rice germplasm,and developing super hybrid rice.