Particular characteristics of soil microbial communities in forest stands infected with Heterobasidion parviporum and Armillaria spp.

摘要

We compared soil microbial communities of forests infected with Heterobasidion parviporum and Armillaria spp. with soils of healthy forests using conventional plating and molecular methods. Plate counts from the soils of the infected forests reflected a significant decrease of the number of cultivable filamentous fungi (CFF) and a slight decrease of the total number of cultivable microorganisms. The diversity of CFF was reduced in the stands infected with H. parviporum. In the stands infected with Armillaria spp. the diversity of CFF and relative abundance of cultivable Trichoderma spp. was even higher than in healthy forest stands. Quantitative PCR revealed increased concentrations of total fungal DNA and Trichoderma spp. DNA in the soil of H. parviporum infected stands. In Armillaria spp. infected stands the total concentration of fungal DNA was decreased, but relative amount of Trichoderma spp. DNA was increased. No significant differences in the species diversity of fungi in the soil were found by ARDRA.