Determination of ethanol in alcoholic drinks using an enzyme biosensor containing alcohol dehydrogenase

摘要

The enzyme biosensor has been developed based on screen-printed carbon electrode (SPCE) containing rhodium dioxide as a mediator and alcohol dehydrogenase as a biocatalyst. Enzyme was immobilized on the electrode surface by electropolymerization of m-phenylenediamine. Resulting biosensor have been tested in model samples of ethanol. In such applications, the biosensor showed a linear response in the range of 15 – 120 g L~(-1) ethanol with a detection limit of 3.3 g L~(-1) (3 σ) and a response time of 19 s. The sensor was applied for analyses of selected alcoholic drinks with very good results.