Discrimination between Transgenic and Non-transgenic Tomatoes with Different Maturities Using Chemometrics and Visible/near-infrared Spectra

摘要

Visible/near-infrared (Vis/NIR) spectroscopy combined with chemometics methods was used to examine the feasibility to non-destructively distinguish transgenic tomatoes from non-transgenic tomatoes with different maturities. Two hundred and five tomatoes were scanned in the Vis/NIR diffuse reflectance mode. Principal component analysis (PCA) and discriminant analysis (DA) were applied to classify tomatoes with different genes. To improve the result, different spectral pre-treatments including multiplicative scattering correction (MSC) and Standard normal variate (SNV) were used. The results show the potential of Vis/NIR spectroscopy to discriminate transgenic tomatoes from non-transgenic ones at the same maturity with accuracy up to 95.61%. At the same time, Vis/NIR spectroscopy can be used to classify green and red tomatoes, no matter what variety the tomato was. However, transgenic and non-transgenic tomatoes with different maturities can not be recognized well. It can be concluded that the recognition accuracy is affected by pigment.