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Synthesis of Carbon Nanotube and Its Potential Application in Protein Purification by Using Skim Latex Serum

机译:用脱脂乳胶血清合成碳纳米管及其在蛋白质纯化中的潜在应用

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Carbon nanotubes (CNTs) have been synthesized by a gas phase double stage chemical vapor deposition (DS-CVD) technique using acetylene (C_2H_2) and hydrogen (H_2) as precursor gases. The morphology and the structure of CNTs were characterized using field emission scanning electron microscope (FSEM) and transmission electron microscope (TEM). The CNTs produced were purified and functionalized by using covalent functionalization methods. The functionalized CNTs were used as column chromatographic media for skim latex protein purification. In this process, pH and ionic strength were optimized to achieve higher efficiency in protein purification.
机译:通过使用乙炔(C_2H_2)和氢气(H_2)作为前体气体,通过气相双级化学气相沉积(DS-CVD)技术合成了碳纳米管(CNT)。使用现场发射扫描电子显微镜(FSEM)和透射电子显微镜(TEM)表征CNT的形态和结构。通过使用共价官能化方法纯化和官能化产生的CNT。官能化CNT用作柱色谱介质,用于脱脂乳胶蛋白纯化。在该方法中,优化了pH和离子强度,以实现更高的蛋白质纯化效率。

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