Chip-Based Energy Transfer System between Quantum dots and Gold Nanoparticles for Analysis of Protein Glycosylation

摘要

We demonstrate a chip-based detection of protein glycosylation by using the energy transfer between quantum dot (QDs) and gold nanoparticles (AuNPs). Our system relies on modulations in the energy transfer between the nanoparticles on a surface; that is, the photoluminescence (PL) of lectin-coated QD (energy donor) immobilized on a glass slide is quenched by carbohydrate-coated AuNPs (energy acceptor), and the presence of the glycoprotein causes the increase of the PL of QD. As a model system, Concanavalin A-coated QD (ConA-QD) and dextran-coated AuNP (Dex-AuNP) were employed to detect the mannosylated proteins, showing that the linear signals were observed for the concentration as well as the number of glycan moiety of the glycoprotein. Our system will be promising for analysis of glycoproteins with high selectivity and sensitivity in a multiplexed and high-throughput manner.