Expression of Suppressors of Cytokine Signaling-3 in Helicobacter pylori-Infected Rat Gastric Mucosal RGM-1 Cells

摘要

Our previous studies show that Helicobacter pylori (H. pylori) induces oxidative stress and the expression of proinflamma tory cytokines in gastric epithelial cells. H. pylori induces the expression of molecular chaperones and proteins involved in protein-folding ma chinery as a defense mechanism against cellular stress. The suppressors of cytokine signaling (SOCS) are known as negative regulators of ma jor immune signal pathways. The purpose of this article is to determine whether H. pylori in a Korean isolate (HP99) induces the expression of SOCS in rat gastric mucosal RGM-1 cells as a defense mechanism. As a result, HP99 induced SOCS-3 expression time-dependently in RGM-1 cells. SOCS-1 was not expressed while SOCS-2 expression was not changed by HP99 infection in RGM-1 cells. SOCS-3 might have a de fensive role in H. pylori-infected gastric mucosal cells. Further study by manipulating SOCS-3 gene should be performed to investigate the phys iological meaning of SOCS-3 induced by H. pylori in gastric mucosal cells.