Sparger and Surface Gas Transfer for Cell Culture Bioreactors

摘要

The impact of bioreactor and sparger scale-up on gas transfer properties was investigated using four geometrically similar bioreactors ranging in size from 15-L to 2500-L total volume with the same sintered metal frit (microporous) sparger design. The mass transfer coefficients for both oxygen and carbon dioxide were measured at each scale at various flow rates. Scale did not directly affect gas transfer properties. Of the four bioreactors investigated, only the 200-L bioreactor had significantly different (higher) values for the oxygen and carbon dioxide sparger mass transfer coefficients.