Cloning and Sequence Analysis of Gene Fragment of Major Allergenic Protein in Tartary Buckwheat

摘要

The partial gene of major allergenic protein in tartary buckwheat was amplified from the total RNA of tartary buckwheat using RT-PCR and 5'-rapid amplication of cDNA ends (5'-RACE) methods. The gene sequence consisted of 1005 nucleotides and a 960 bpopen reading frame (ORF) which encoded a protein of 320 amino acids residues. Results of homology analysis revealed that the cDNA sequence shared 90% homology with the major allergenic storage protein and legumin-like protein of common buckwheat. The deduced ammo acid sequence shared 84% and 76% homology with the legumin-like 13S storage protein and the major allergenic storage protein of common buckwheat respectively. The obtainment of gene fragment of major allergenic protein in tartary buckwheat should lay an important foundation for further study of the tartary buckwheat allergen.