Effect of Hydroxyapatite-Tricalcium Phosphate Micro-particles on the Cellular Interaction of In Situ Hyaluronic Acid-Poly(ethylene oxide) Hydrogel

摘要

In vitro cell behaviors of calvarial osteoblasts (MC3T3) were evaluated by seeding them on both the surface and inside of in situ hyaluronic acid-poly(ethylene oxide) (HA-PEO) hydrogel, either after or before incorporation of mixture micro-particles of hydroxyapatite-beta-tricalcium phosphate on/inside the hydrogel, respectively. Cellular behaviors such as adhesion and proliferation on the surface and inside the gel were evaluated with light microscopy and a microplate reader by focusing on the interactions of cell-HA-PEO as well as cell-hydroxyapatite-tricalcium phosphate micro-particle surface in the gel. Cell adhesion and spreading seemed to be enhanced by supplying the micro-particles to the inside the HA-PEO hydrogel, compared to the results of the HA-PEO hydrogel itself.