Preparation and characterization of Pluronic/TPGS mixed micelles for solubilization of camptothecin

摘要

To increase the solubility and bioavailability of poorly soluble anticancer drug camptothecin (CPT), CPT-loaded mixed micelles were prepared. METHODS. Mixed micelles made of Pluronic P105 (P105) and D--tocopheryl polyethylene glycol 1000 succinate (TPGS) were prepared by film formation method and poorly soluble anticancer drug CPT was incorporated into the mixed micelles. The amount of CPT in the micellar phase was determined by the reversed phase-HPLC. The cytotoxicity of the CPT-loaded mixed micelles against MCF-7 cancer cell in vitro was assessed by the MTS colorimetric assay for the dehydrogenase activity of viable cell. RESULTS. The solubility of CPT by the mixed micelles was more than that of the free drug. The cytotoxicity of the CPT-loaded mixed micelles against MCF-7 cancer cell in vitro was remarkably higher than that of the free drug. CONCLUSIONS. Mixed micelles made of Pluronic P105 and TPGS may represent potential delivery system for sparingly soluble anticancer drugs.